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Figure 3 | Molecular Brain

Figure 3

From: The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release

Figure 3

nPKCε expression is modulated by synaptic activity. (A) Western blot analysis of nPKCε and pnPKCε immunoreactivity levels in synaptic membrane in adult stimulated muscles with (ES + C) and without (ES) muscle contraction. (B) shows Western blots of nPKCε to compare the effect of the method to abolish muscle contraction (cut and μ-CgTx GIIIB (μCTX)). Quantitative data eliminate any possible artifact due to the method used to abolish muscle contraction. There are no significant differences between ratios μCTX vs Cut. The Western blot shows: (1) levels of nPKCε in non-stimulated muscles where no method was performed to abolish muscle contraction (Ctr), in cut muscles (Cut) and in muscles where muscle contraction was abolished by μ-CgTx GIIIB (μCTX); (2) nPKCε in electrically stimulated intact muscles where muscle contraction was abolished by μCgTx GIIIB (ES,μCTX); and (3) nPKCε in stimulated muscles resulting in contraction (ES + C). Experiments were performed using diaphragm muscle.

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Molecular Brain

ISSN: 1756-6606