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Figure 4 | Molecular Brain

Figure 4

From: The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release

Figure 4

Electrical activity-induced muscle contraction promotes changes in nPKCε isoform through trkB activity. Western blot analysis of nPKCε and pnPKCε immunoreactivity levels in adult contracting muscles (ES + C) in the presence or not of the TrkB inhibitor Ana12 (A) or anti-TrkB antibody (47/TrkB) (B). In all the muscles, the phrenic nerve was stimulated at 1 Hz for a 30-minute period and muscle contraction was not prevented. The quantitative histograms show the changes in (ES + C) with TrkB inhibitor with respect to (ES + C) and normalized with actin. The data are mean ± SD values from at least 5 independent experiments. *p < 0.05. Experiments were performed using diaphragm muscle.

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Molecular Brain

ISSN: 1756-6606