Skip to main content
Figure 6 | Molecular Brain

Figure 6

From: The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release

Figure 6

nPKCε regulates phosphorylation of MARCKS in adult skeletal muscle, in an activity-dependent way. (A) shows the Western blotting analysis of pMARCKS and Hsp70 in control (Ctr) and in stimulated muscles (ES). (B) shows the effects of εV1-2 incubation and electrical activity (V + ES) on the amount of pMARCKS. (C) shows the Western blotting analysis of nPKCε and pnPKCε in several conditions of stimulation: in control (Ctr), in stimulated muscles (ES), in stimulated muscles previously incubated with the blocking peptide (V + ES), in stimulated muscles previously incubated with the inactive peptide (Vs + ES). In all these experiments, the phrenic nerve was stimulated at 1 Hz for a 30-minute period and muscle contraction was prevented. The data are means ± SD values from at least 5 independent experiments and are normalized with actin. *p < 0.05. Experiments were performed using diaphragm muscle.

Back to article page

Molecular Brain

ISSN: 1756-6606