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Figure 7 | Molecular Brain

Figure 7

From: The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release

Figure 7

Muscle contraction enhances phosphorylation of MARCKS through TrkB. (A) shows the Western blotting analysis of pMARCKS in synaptic membrane in adult stimulated muscles with (ES + C) and without (ES) muscle contraction. (B) shows the Western blot analysis of pMARCKS immunoreactivity levels in adult contracting muscles (ES + C) in which has been selectively inhibited TrkB activity using the anti-TrkB antibody (47/TrkB) or the fusion protein trkB-Fc (to chelate endogenous BDNF/NT-4). In all these experiments, the phrenic nerve was stimulated at 1 Hz for a 30-minute period and muscle contraction was prevented (ES) or not (ES + C). The data are means ± SD values from at least 5 independent experiments and are normalized with actin. *p < 0.05. Experiments were performed using diaphragm muscle.

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Molecular Brain

ISSN: 1756-6606