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Figure 8 | Molecular Brain

Figure 8

From: The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release

Figure 8

PMA-induced PKC-coupling to ACh release potentiation involves nPKCε. The histogram in (A) indicates that ACh release is increased by incubation with PMA. The dose-dependence analysis of the effect of the εV1-2 shows that the previous block of the nPKCε reduces (1 and 10 μM) and even abolishes (100 μM) the PMA potentiation of ACh release. For each kind of experiment: n = 5 adult muscles and a minimum of 15 fibers per muscle. *means p < 0.05. (B) shows the raw data of superimposed EPPs in the conditions described. Thus, the integrity of nPKCε seems a necessary condition if the PMA-induced transmitter release potentiation is to be fully expressed. Vertical bars: left, 10 mV; right, 5 mV. Horizontal bars: 4 ms. Experiments were performed using diaphragm muscle.

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Molecular Brain

ISSN: 1756-6606