Figure 4

Effect of CaMKIIα overexpression on mGluR1a- and mGluR5a- -stimulated ERK1/2 phosphorylation. A) Shown are representative immunoblots for FL-mGluR1a expression, p-ERK1/2 activity and total-ERK1/2 expression in HEK 293 cells transiently transfected with 3 μg of pcDNA3.1 encoding FLAG-mGluR1a along with 0.5 μg of plasmid cDNA encoding either GFP, GFP-CaMKIIα or GFP-CaMKIIα-T286A in response to 50 μM quisqualate treatment for 0, 5 and 10 min. Bar graph shows the densitometric analysis of ERK1/2 phosphorylation normalized to both basal and total ERK1/2 protein expression. Data represents the mean ± SD of five independent experiments. *P < 0.05 versus GFP transfected control cells. B) Shown are representative immunoblots for, FL-mGluR5a expression, p-ERK1/2 activity and total-ERK1/2 expression in HEK 293 cells transiently transfected with 3 μg of pcDNA3.1 encoding, FL-mGluR5a along with 0.5 μg of plasmid cDNA encoding either GFP, GFP-CaMKIIα or GFP-CaMKIIα-T286A in response to 50 μM quisqualate treatment for 0, 5 and 10 min. Bar graph shows the densitometric analysis of ERK1/2 phosphorylation normalized to both basal total ERK1/2 protein expression. Data represents the mean ± SD of five independent experiments. *P < 0.05 versus GFP transfected control cells.