Aβ42 oligomers increase CaMKIIα co-immunoprecipitation with mGluR5a. A) HEK 293 cells were transiently transfected with 2 μg of pcDNA3.1 encoding FLAG-mGluR5a and 0.5 μg of plasmid cDNA encoding GFP-CaMKIIα. HEK 293 cells were treated with 200 nM of Aβ42 oligomer for 0, 2, 5, 15 and 30 min. B) The bar graph shows the densitometric analysis of the relative co-immunoprecipitation of GFP-CaMKIIα with FL-mGluR5a following Aβ42 oligomer treatment normalized to total mGluR5a protein expression. Data represents the mean ± SD of 5 independent experiments. *p < 0.05 versus untreated cells.