BAPTA inhibited autophagy activation and neuroprotection induced by IPC in PC12 cells. (A) BAPTA abolished IPC induced neuroprotection in PC12 cells. Cells were incubated with BAPTA 60 min before the onset of IPC. Twelve hours after IPC, the cells were subjected to OGD for 10 h and cell viability was examined with CCK-8 kit. (B)-(D) The cells were incubated with BAPTA 60 min before the onset of IPC. Then the cells were harvested 12 h after IPC and subjected to Western blot analysis. (B) BAPTA reduced the expression of GRP78 protein. (C) BAPTA reduced LC3II/LC3I ratio. (D) BAPTA reduced the expression of Beclin1 protein. Bar represents mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group; $$ P < 0.01 compared with the OGD group; % P < 0.05 compared with the IPC + OGD group; # P < 0.05, ##P < 0.01 compared with the IPC group. B (L) = BAPTA 0.125 μM; B (M) = BAPTA 0.5 μM; B (H) = BAPTA 2 μM.