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Figure 3 | Molecular Brain

Figure 3

From: The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation

Figure 3

RNAi-resistant PTEN rescues the effect of PTEN knockdown. (A) Schematic illustration of lentiviral-based PTEN wild type, G129E, Y138L rescue constructs. (B) 293 T cells were transfected with vector, HA-PTEN-WT, HA-PTEN-WT-rescue, HA-PTEN-G129E-rescue, HA-PTEN-Y138L-rescue resulted in the expression of a 55 kDa band corresponding to the PTEN protein, as seen by western blotting. (C) The data show the Tuj1 positive cells in control, PTEN RNAi, PTEN RNAi + HA-PTEN-(WT, G129E, Y138L)-rescue conditions respectively. (D) NSCs transduced with GFP, PTEN RNAi, or PTEN RNAi + HA-PTEN-(WT, G129E, Y138L)-rescue constructs were labeled with an antibody against the neuronal marker Tuj1. Scale bar = 40 μm. Data are expressed as mean ± SD. **P < 0.01, ***P < 0.001 (n > 800 cells per group from each experiment).

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Molecular Brain

ISSN: 1756-6606