Figure 7

Bergmann glia specification was not abolished in Sox9-null cerebellum. (A) Real-time PCR of the oligodendrocyte marker Olig2 and astrocyte marker S100 showed that gliogenesis was not perturbed in Sox9 mutant (p = 0.2764 and p = 0.7551 respectively). (B-C) At E13.5, BLBP expression that marked the early-born radial glial cells which function as the cerebella neural stem cell pool, spanning the entire VZ in both the control and Sox9 mutant. Expression of BLBP persisted in the cerebellum throughout embryonic development as indicated by both coronal sections (D-E) and sagittal sections (F-I). (F’-I’) Particularly strong BLBP signal in the VZ where soma of radial glia resides was seen in both control and mutant cerebella. Filamentous BLBP immunoreactivity of the glial scaffold was not disrupted in the Sox9-null cerebellum. (F”-I”) Robust BLBP expression representing prospective Bergmann glia could be observed in the PCL of Sox9 mutants (broken line), with the glial endfeet which terminated at the pial surface equally conspicuous in the control and Sox9 mutant.