Figure 8

Normal gliogenesis in the Sox9 mutant cerebellum as shown by immunofluorescence staining on cerebellar sagittal sections. (A-B) Robust expression of EAAT1 at E15.5 in both control and Sox9 null mutants further confirmed the intactness of radial glial scaffold. (C-D) At E18.5, EAAT1 expression was found in the PCL, similar to that of BLBP. (C’-D’) The glial filaments with EAAT1 immunoreactivity were prominent in the mutants despite inactivation of Sox9. (E-F) The periventricular expression of S100 at the PCL also suggests the presence of astroglia in both the control and Sox9 mutant cerebella. (G-H) The normal expression of GFAP in the Sox9 mutant cerebellum suggested that differentiation of Bergmann glia was intact upon knockout of Sox9, as evident by the robust distribution of GFAP especially at the PCL where Bergmann glia are found (G’-H’). Abbreviations: pcl, Purkinje cell layer; vz, ventricular zone. Scale bars: 100 μm.