Figure 13
Vascular pathologies in Aldh2 -/- mice. Cerebral microvessels from Aldh2 -/- mice exhibit increased HNE adduct formation (A) and age-related increases in Aβ formation (B). Representative dot-blots (HNE, 20 μg protein) and immunoblots (Aβ, 30 μg protein) are from 9 month old animals. Immunoreactive spots or bands were quantitated by densitometry. Data are presented as the mean ± SD (n = 4 mice) and were analyzed by a two-way ANOVA with a Bonferroni post-hoc test. ***, significant difference from wildtype (p < 0.001). ψ ψ ψ, significant age-related differences as indicated (p < 0.001). In aortic ring preparations from 12 month old Aldh2 -/- mice there was a significant decrease in both the potency and maximal relaxation response to the endothelium-dependent vasodilator, acetylcholine (ACh)(C), and a significant increase in the potency of the adrenoceptor agonist, phenylephrine (Phe) (D). Data are presented as the mean ± SD (n = 4 mice) and EC50 values for relaxation or contraction were analyzed by Student’s t-test for unpaired data.