Figure 10

Nr2e1 ^frc/frc Müller glia cells were aberrantly positioned in the INL. Transverse retinal sections from P7 Nr2e1 ^+/+, Nr2e1 ^frc/frc, and chimeric mice were immunostained for SOX-2 and Islet-1/2. a Nr2e1 ^+/+ SOX-2 positive Müller-glia somas (red, bracket) were localized between cholinergic amacrines in the lower INL and ON bipolars in the upper INL, the latter two cell types both labeled with Islet-1/2 (green). Müller-glia somas localized close to the middle of the INL and away from the OPL. b Nr2e1 ^frc/frc Müller-glia somas (red, bracket) intermingled with ON bipolars (green) and localized adjacent to the OPL. c In Wt↔frc chimeras, the mispositioning of the Müller-glia soma was seen only in Nr2e1-mutant cells (EGFP positive, green), which were located closer to the OPL compared to wild-type cells (EGFP negative). Representative images of a 51 % Wt↔Wt and a 58 % Wt↔frc chimeric retina are shown. d,e Magnification of the boxes in (c) showing a region from the (d) Wt↔Wt chimera depicting Müller glia located close to the middle of the INL, and from the (e) Wt↔frc chimera depicting mutant Müller glia located adjacent to the OPL. The dotted lines represent an imaginary boundary above which most wild-type cells localize. Note that in a Wt↔frc chimera, most Nr2e1-mutant cells (EGFP positive) localize under this line close to the OPL and most wild-type cells (EGFP negative) localize above this line and away from the OPL. n = 3 for Nr2e1 ^+/+, n = 3 for Nr2e1 ^frc/frc, n = 4 for Wt↔Wt, n = 4 for Wt↔frc; EPL, Ectopic plexiform layer; GCL, ganglion cell layer; INL, inner nuclear layer; OPL, outer plexiform layer; Hoechst, nuclear counterstain (blue); scale bar = 50 μm