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Table 1 Differential effects of molecular target inhibitions on FP, VSD and IOS signals. Ratio of drug effects on optical signals and population spikes. Ratios were calculated by dividing the change in optical signal amplitude due to drug application (expressed in % of control) by the change in population spike or VSD amplitude (also expressed in % of control). Asterisks indicate significant changes compared to control (P < 0.05 Mann–Whitney U test). NA means not applicable (PS amplitude is 0 mV)

From: Appearance of fast astrocytic component in voltage-sensitive dye imaging of neural activity

Molecular target (inhibitor)

VSD/PS ratio

IOS/PS ratio

IOS/VSD ratio

IOS/VSD ratio

IOS/VSD ratio

CA1

CA1

CA1

CA3

DG

Control

0.90 ± 0.05

1.05 ± 0.02

1.09 ± 0.05

1.06 ± 0.06

1.03 ± 0.05

GluR receptors (CNQX + APV)

NA

NA

1.78 ± 0.30*

0.55 ± 0.22*

0.36 ± 0.13*

EAAT2 (DHK)

0.53 ± 0.04*

0.53 ± 0.08*

0.77 ± 0.16

1.98 ± 0.33*

5.68 ± 2.02*

KCC2 and NKCC1 (furosemide)

0.81 ± 0.10

0.43 ± 0.07*

0.45 ± 0.06*

0.46 ± 0.04*

0.32 ± 0.08*

NKCC1 (bumetadine)

0.93 ± 0.06

1.17 ± 0.07

0.94 ± 0.04

1.05 ± 0.05

1.09 ± 0.13

KCC2 (VU0463271)

0.53 ± 0.08*

0.53 ± 0.16*

1.00 ± 0.10

1.00 ± 0.10

0.91 ± 0.10

Anion channels (DIDS)

0.62 ± 0.05*

0.41 ± 0.07*

0.67 ± 0.08*

0.59 ± 0.08*

0.61 ± 0.11*

Kir4.1 (BaCl2)

0.65 ± 0.06*

0.8 ± 0.09*

1.29 ± 0.12

1.5 ± 0.12*

3.03 ± 0.75*

Na+/K+ ATPase (ouabain)

0.71 ± 0.09

0.63 ± 0.15

1.13 ± 0.12

1.10 ± 0.10

1.39 ± 0.10*

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Molecular Brain

ISSN: 1756-6606