- Categorization was done according to the effect of age, BD, cortical region and the process that a particular protein is involved in. The first two ‘age-regulated’ columns visualize the presence and direction of difference (up or down) between the 2N and 4N group within central and peripheral area 17. The next two columns show the effect of age when reared under BD conditions, indicating the presence and direction of difference between the 2BD and 4BD group. In the next four columns, proteins were classified as ‘2BD-regulated’ or ‘4BD-regulated’ when their expression differed between a particular BD group and its age-matched normal control, or when there was a difference between the central and peripheral region in a BD group but not in its age-matched normal control group (+) or vice versa (−). Between 4BD and 2N kittens, the peripheral region was molecularly similar for 97% (except protein in row 18), the central region was similar for 69% (25 proteins, marked in bold italics) and all other proteins showed higher expression in 4BD as compared with 2N. For CRMP2 and CRMP4 spot numbers that do not contain other proteins are in bold. Abbreviations: C: central; P: peripheral; o: outgrowth; s: synaptic transmission; c: clathrin-mediated endocytosis; m: energy metabolism; mR: mRNA metabolism and transport; ↑ – upregulation, or ↓– downregulation. All presented differences are significant, p < 0.05
Molecular Brain