Fig. 2

The crucial role of NR2B-containing NMDARs in the magnesium-induced restoration. a The protein expression levels of NR2A (Ctl, 11 mice; Mg, 13 mice) and NR2B (Ctl, 12 mice; Mg, 13 mice) in V1b of control and magnesium-treated mice. Using two-tailed Student’s t test, P = 0.0002 for NR2A, P = 0.00002 for NR2B. Error bars, SEM. b Schematic of the experimental procedure (top) and a summary of the CBIs in each group with a local infusion of NR2B antagonist (Ro, Ro 25-6981), NR2A antagonists (PPPA/TCN; PPPA, light green; TCN 201, dark green) or vehicle (bottom). The drugs were locally infused to V1b of magnesium-treated mice using an osmotic minipump one day prior to MD. P < 0.01 using a one-way ANOVA followed by Tukey’s post hoc test. c Schematic of the experimental procedure (top) and a summary of the CBIs in magnesium-treated mice with or without Ro 25-6981 (bottom). Both magnesium and Ro were removed prior to the onset of MD. The gray area represents the normal CBI level. The horizontal bar represents the mean value; each symbol represents one animal. P = 0.005 using two-tailed Student’s t test. **P < 0.01