Fig. 2
From: Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity
Protein expression levels and cellular localization of ETB receptor following SE. a Western blot image of ETB receptor in the hippocampus. ETB receptor expression is gradually increased at 6 h – 3 days after SE. b Quantitative values (mean ± S.E.M) of ETB receptor expression level in the hippocampus, based on western blot (n = 10 per each group). Significant differences from non-SE animals, *p < 0.05. c-f Representative photographs of ETB receptor and NeuN in the CA1. Following SE, ETB receptor expression is gradually elevated in CA1 pyramidal cells. Three days after SE, NeuN immunostains show neuronal damage. Arrows indicate neurons containing ETB receptor expression. Bar = 50 μm. g-h Representative photographs of ETB receptor and GFAP. ETB receptor expression is weakly observed in a few astrocyte in non-SE animals. One day after SE, ETB receptor expression is elevated in astrocyte. Arrows indicate astrocytes containing ETB receptor expression. Bar = 50 μm