Fig. 3
From: Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity
Effect of BQ788 and Cav-1 peptide on vasogenic edema, BBB and neuronal damage following SE. a Quantitative values (mean ± S.E.M) of nitrate/nitrite (NO products) levels in the hippocampus at 4 h after SE (n = 10 per each group). Significant differences from non-SE animals, *p < 0.05. b Representative photographs of vasogenic edema formation at 1 day after SE. Both BQ788 and Cav-1 peptide treatments attenuate SE-induced vasogenic edema. Bar = 400 μm. c Representative photographs of SMI-71 in the CA1 at 1 day after SE. In non-SE animals, SMI-71 expression is detected in most of vessels. Following SE, SMI-71 immunoreactivity is decreased. Both BQ788 and Cav-1 peptide treatments attenuate SE-induced BBB breakdown. Bar = 50 μm. d FJB-positive neuronal damage in the CA1 at 3 days after SE. BQ788 infusion attenuates SE-induced neuronal damage, while Cav-1 peptide infusion does not affect SE-induced neuronal damage. Bar = 100 μm. e Quantitative values (mean ± S.E.M) of IgG and SMI-71 expression in the hippocampus (n = 10 per each group). Significant differences from vehicle, *p < 0.05. f Quantitative values (mean ± S.E.M) of FJB-positive degenerating neurons (n = 10 per each group). Significant differences from vehicle, *p < 0.05