Fig. 1

In situ hybridization analysis of indomethacin-induced gene regulation. Representative photomicrographs of hippocampal sections hybridized with radiolabeled riboprobes. Quantified expression of the indicated layers is shown by bar graphs on the right. Results are expressed as optical density values relative to vehicle treatment and are the mean ± SEM of four separate animals, each analyzed in duplicate brain sections. a Downregulation of Cox-2 is prominently seen in the hippocampus but not the cortex. Hippocampal cell layers are identified in the lower panel, showing reduction in the dentate gyrus (DG), CA1 and CA3 regions. b NPY downregulation is evident in all the brain regions examined, hippocampus and cortex. Expression in the inner and outer cortical layers were quantitate using boxes. c Marked SGK upregulation is seen in the white matter corpus callosum (CC) and lower levels of induction in the stratum lacunosum moleculare (SLM), hilus and cortex