Fig. 5

Effects of NB001 on thermal and joint hyperalgesia. Thermal hyperalgesia was examined on the last experimental day for ankle arthritis mice. a CFA caused thermal hyperalgesia in the ipsilateral hindpaw but not the contralateral hindpaw. NB001 administration (3 mg/kg, i.p.) significantly inhibit thermal hyperalgesia. b Joint hyperalgesia was assessed by the joint flexion test on the last experimental day and expressed as the numbers of vocalization caused by bending and extension of the joint (five times in each direction). The mice exhibited evident ankle joint hyperalgesia following CFA injection in the ipsilateral joint. c The mice exhibited markedly knee joint hyperalgesia following CFA injection in the ipsilateral joint. NB001 significantly (3 mg/kg, i.p.) attenuated both ankle (b) and knee joint hyperalgesia (c) indicted by reduction of vocalized numbers in joint flexion tests. n = 8 in each group. ^* p < 0.05, ^** p < 0.01 compared to the control group; ^## p < 0.01 compared to the CFA group