Fig. 3

Gene expression analysis of epigenetic factors following global ischemia at several times preceding neuronal death. Quantitative PCR analysis of (a) Dnmt1 and Dnmt3a, (b) Hdac1, Hdac2 and Hdac3, (c) Nr3c1 and (d) Gadd45β, at 6, 12 and 24 h after reperfusion. mRNA values, expressed as copy number, were normalized to β-actin and are means ± S.E.M. of 3-5 individual determinations; One-way ANOVA + Fisher's LSD. In (a) Dnmt3a at 6 h: F_(3,15) = 9.3, p < 0.05; In (b) Hdac1 at 6 h: F_(3,17) = 4.8, p < 0.05; at 24 h: F_(3,15) = 3.8, p < 0.05; Hdac2 at 12 h: F_(3,13) = 4.6, p < 0.05; Hdac3 at 6 h: F_(3,17) = 2.9, p < 0.05; in (c) Nr3c1 at 6 h: F_(3,17) = 4.3, p < 0.05; Nr3c1 at 12 h: F_(3,14) = 5.165 p < 0.05 and in (d) Gadd45β at 6 h: F_(3,17) = 48.4, p < 0.0001, at 12 h: F_(3,13) = 8.6 p < 0.005. Post-hoc analysis: p < 0.05 vs. the respective values obtained in sham operated rats (*); or vs. the respective values obtained in the CA1 region (#)