Fig. 1
From: Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells
Rapid, efficient and simple motor neuron differentiation from human pluripotent stem cells. a Schematic presentation of motor neuron differentiation from hPSCs. b Time-course analysis of the expression of PAX6, SOX1, and NGN2 in EBs via quantitative RT-PCR analysis. Control, 0.09 % DMSO. DS, dorsomorphin and SB431542. DSB, dorsomorphin, SB431542 and BIO. n = 4, mean ± SEM. *, p < 0.05 (Student’s t test). c Time-course analysis of the expression of PAX6, SOX1, NGN2, OLIG2, NKX2.2, HB9, and ISL-1 mRNA in EBs and during monolayer motor neuron (MN) differentiation. n = 4, mean ± SEM. d Immunocytochemical analysis of HB9, ISL-1, and βIII-Tubulin 1 week after monolayer differentiation. Scale bar, 100 μm. e Quantitative analysis of the number of the HB9+ and ISL-1+ cells. n = 3, mean ± SEM