Fig. 4
From: Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells
Derivation of motor neurons from hiPSCs. a Immunocytochemical analysis of motor neurons derived from 201B7, TIGE-9 and YFE-16 for HB9, ISL-1, and βIII-Tubulin after 2 weeks of monolayer differentiation and ChAT after 4 weeks of monolayer differentiation. Scale bar, 100 μm. b Quantitative analysis of the number of the cells positive for motor neuron markers in cultures derived from each hiPSC clone. n = 3, mean ± SEM. c Time-course analysis of the expression of HB9, ISL-1, and ChAT during monolayer differentiation of hiPSC-derived motor neurons via quantitative RT-PCR. n = 3, mean ± SEM. d Western blot analysis of the expression of the HB9, ISL-1, and ChAT proteins during monolayer differentiation of hiPSC-derived motor neurons. B7, 201B7. T9, TIGE-9. Y16, YFE-16. e Quantitative analysis of the expression of the HB9, ISL-1, and ChAT proteins through densitometry using ImageJ. Protein expression levels are normalized to β-Actin. n = 3, mean ± SEM. *, p < 0.05, (Student’s t test)