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Fig. 3 | Molecular Brain

Fig. 3

From: Huwe1 interacts with Gadd45b under oxygen-glucose deprivation and reperfusion injury in primary Rat cortical neuronal cells

Fig. 3

The co-expression of Huwe1 and Gadd45b existed in cortex neuron cells. Cells were prepared to incubate with anti-Huwe1 (Green) and anti-Gadd45b (Red) antibodies for double immunofluorescent labeling staining. DAPI (blue) was used for cell nucleus staining. The co-expression of Huwe1 and Gadd45b obviously increased at 24 h after reperfusion (b), compared to normal neuron cells controls (a). Inhibition of Huwe1 (d) increased the expression of Gadd45b, compared to the lentivirus-GFP-scramble control group (c) at 24 h. (e-h) showed that the co-localization of Huwe1 and Gadd45b by laser confocal scanning microscopy. The co-localization of Huwe1 and Gadd45b is mainly around the cell nucleus (f-g). i-j The average optical density results of Huwe1 and Gadd45b. *p < 0.05 versus control, # p < 0.05 versus LV-GFP-ctrl. Scale bar = 10 μm

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Molecular Brain

ISSN: 1756-6606