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Fig. 5 | Molecular Brain

Fig. 5

From: Huwe1 interacts with Gadd45b under oxygen-glucose deprivation and reperfusion injury in primary Rat cortical neuronal cells

Fig. 5

Inhibition of Gadd45b decreased the expression of BDNF at 24 h after reperfusion. a Western blot assay of BDNF expression at the indicated time points after OGD/R. b Neuron cells were infected with lentivirus shRNA-Huwe1 and/or lentivirus shRNA-Gadd45b, followed by OGD and reperfusion 24 h. ShRNA-Gadd45b (LV-shGadd45b) decreased the expression of BDNF, compared to lentivirus scramble control group (LV-ctrl). However, shRNA-Huwe1 (LV-shHuwe1) increased the expression of BDNF. c qPCR result of BDNF after treatment with shRNA-Huwe1 or shRNA-Gadd45b. d The methylation results of BDNF IXa by BSP assay. The BDNF IXa included six CpG islands. Inhibition of Huwe1 or Gadd45b changed the level of BDNF IXa methylation at 24 h after reperfusion. Tubulin was used as an internal control for western blot; GADPH was used as an internal control for qPCR; *p < 0.05 versus control, # p < 0.05 versus LV-GFP-ctrl or LV-ctrl

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Molecular Brain

ISSN: 1756-6606