Fig. 8

The morphological features of apoptosis were monitored by fluorescence microscopy after staining with Hoechst 33342. Neuron cells were infected with lentivirus shRNA-Huwe1 or shRNA-Gadd45b, followed by OGD and reperfusion 24 h. The dye Hoechst 33342 was introduced to characterize the cells nucleic and emits blue fluorescence at 480 nm. Cells that exhibited reduced nuclear size, chromatin condensation, intense fluorescence, and nuclear fragmentation were considered to be apoptotic (white arrow). OGD/R induced neuron cells apoptosis at 24 h (b), compared to normal neuron cells (a). Treatment with shRNA-Gadd45b increased the number of apoptotic cells (c). Treatment with shRNA-Huwe1 decreased the number of apoptotic cells (d). Scale bar = 10 μm