Fig. 4

Differential sensing mechanism of nutritive sugars. a-c Volume of 100 mM D-/L-glucose consumed in a meal, by flies with indicated genotypes starved for 12 and 36 h (n = 24-46). d-f Volume of 100 mM D-/L-fructose consumed in a meal, by flies with indicated genotypes starved for 12 and 36 h (n = 19-36). g A working model. Starved flies can assess the nutritional value of food (i.e. the presence of D- vs. L-glucose and D- vs. L-fructose) within 20–30 s and modulate food ingestion accordingly. SLC5A11 and Gr43a are required for the food nutrient assessment, respectively. Sample size for each data set was summarized in Tab. S1. Error bars represent SEM. ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Student’s t-test was used for pair wise comparisons. One-way ANOVA followed by Bonferroni post hoc test was used for comparisons for more than 2 groups. Two-way ANOVA (and post hoc test if applicable) was applied for comparisons with more than one variant