Fig. 7
From: Rasd1, a small G protein with a big role in the hypothalamic response to neuronal activation
Lentiviral mediated manipulation of Rasd1 expression in the rat SON alters c-Fos, Nr4a1 and hnAvp expression. a confirmation of Rasd1 protein expression 72 h after viral transduction in mouse neuronal cell line N2a. b lentiviral transduction of MCN in the SON 2 weeks after injection. c rats were injected into SON with lentiviral vectors expressing Rasd1 or eGFP. Two weeks after viral administration rats received a single i.p injection of either IS (0.15 M NaCl) or HS (1.5 M NaCl; 1.5 ml/100 g body weight). Brains were collected 30 min later. Relative expression of c-Fos, Nr4a1, hnAvp and Rasd1 was determined by qPCR. d two weeks after dnRasd1 injection rats were presented with 2% (w/v) NaCl solution in place of drinking water for 7 days. Relative expression of c-Fos, Nr4a1, hnAvp and Rasd1 in SON of dnRasd1 delivered animals compared to control eGFP control. c, Two-way ANOVA; d, independent-sample unpaired Student’s t tests. Scale bars = 100 μm. Con, control; MOI, multiplicity of infection. Values are means + SEM of n = 4−5 animals per group. *p < 0.05, **p < 0.01, ***p < 0.001