Fig. 3

Overexpression of DN-RAB18 (S22N) and suppression of RAB3GAP2 all affect cortical neuronal migration. a Representative coronal section showing migration of transfected neurons 3 d after electroporated at E14.5 with GFP plasmid together with indicated constructs. Scale bar: 100 μm. b Data represent the mean ± SEM and the regional distribution of GFP positive neurons is quantitatively analyzed in the cerebral cortex from at least three brains. c Magnified images of individual electroporated neurons in intermediate zone. A high percentage of migrating DN-RAB18 (S22N) expression neurons stayed at the multipolar stage (arrowhead). Scale bar: 20 μm. d Quantification of the percentages of neurons with uni/bipolar and multipolar morphology. Error bar indicated the SEM of three different brains. e Immunoblot analysis shows that RAB3GAP2 shRNA-2 (shRAB3GAP2-2) is efficient to knockdown FLAG-RAB3GAP2 and endogenous RAB3GAP2 in HEK293T cells and cultured cortical neurons. f Representative coronal section showing migration of transfected neurons 3 d after electroporated at E14.5 with GFP plasmid together with indicated constructs. Scale bar: 100 μm. g Quantification of percentages of GFP positive neurons in different cortical regions. Data represent the mean ± SEM and the regional distribution of GFP positive neurons is quantitatively analyzed in the cerebral cortex from at least three brains. h Magnified images of individual electroporated neurons in intermediate zone. A high percentage of migrating RAB3GAP2 knockdown neurons stayed at the multipolar stage (arrowhead). Scale bar: 20 μm. i Quantification of the percentages of neurons with uni/bipolar and multipolar morphology. Error bar indicated the SEM of three different brains. ^*** p < 0.001, ^** p < 0.01 and ^* p < 0.05 versus control (or pSUPER); t test