Fig. 6

N-cadherin lysosomal degradation is enhanced in RAB18 deficient neurons. a Representative images of cortical neurons stained for RAB7 (red), RAB18 (green) and DAPI (blue). Scale bar: 5 μm. b Immunoblot analysis of cortical neurons infected with indicated plasmids with the indicated antibodies. c The graph indicates the ratio of N-cadherin / Tuj1 in b. Knockdown of RAB18 significantly decreased the total N-cadherin protein level of neurons. d Immunoblot analysis of cortical neurons infected with indicated plasmids with the indicated antibodies. Leupeptin (50 μM) was done 12 h before harvesting. e The graph indicates the ratio of N-cadherin / Tuj1 in d. Knockdown of RAB18 has no significant differences in the total N-cadherin protein level of leupeptin treated neurons. Data represent the mean ± SEM and the experiment was performed at least four times. ^*** p < 0.001 versus control