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Fig. 6 | Molecular Brain

Fig. 6

From: Electrophysiological behavior of neonatal astrocytes in hippocampal stratum radiatum

Fig. 6

Discrete electrical coupling among neonatal astrocytes. a Dual patch recording from a pair of neonatal astrocytes in CA1 stratum radiatum. The recorded cells (DIC, left) were selected based on SR101 staining (right). B1, B2. A pair of electrically coupled (top), and uncoupled astrocytes (bottom), respectively. The current steps, shown in inset, were alternately delivered to the stimulated cell (C stim.) that induced transjunctional voltage (V transjunction) only in the recipient cell (C reci.) of the coupled pair (top). c The coupling occurred in either VRA-VRA, PA-PA homotypic or VRA-PA heterotypic pairs. Likewise, the uncoupled pairs were not associated with either VRA or PA phenotypes. d Nearly all the PAs were coupled compared to 40 % of uncoupled VRAs. e The cell-to-cell pair distance was not associated the occurrence of cell coupling. f The recording configuration for coupling coefficient (CC) measurement, the V COM voltages, ranging from −220 to +60 mV, were delivered to the C stim. in voltage clamp mode, whereas the V transjunction was recorded in the C reci. in current clamp mode. g The CC values varied insignificantly throughout the test V COM voltages (n = 9, P > 0.05). h The variation of CC values was independent of cell-to-cell pair distances. i The CC varied substantially among all the recorded pairs (n = 13)

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