Fig. 4
MB expression of a rut + transgene is sufficient to restore LTE formation and increase cAMP synthesis in chico mutants. a, b MB expression of the rut + transgene restores LTE in chico mutants. a Typical AL-induced Ca2+ responses before (pre) and 5 min after (post) AL + AFV stimulation in chico 1 , UAS-rut/chico 1 ; UAS-G-CaMP/+; OK107/+ (rut rescue) brains. Scale bar, 5 μm. b Summary of Ca2+ responses in control, chico 1 and rut rescued flies. c, d Typical traces (left) and average maximal responses (right) of AL-induced (c) and AFV-induced (d) Ca2+ responses in the MBs of chico 1 (control) and rut rescue brains. N = 6 for all data in a–d. e, f MB expression of a rut + transgene increases cAMP synthesis in chico 1 mutants upon forskolin stimulation. e Typical FRET images and time course (normalized CFP/YFP) upon forskolin stimulation in control (UAS-Epac1-camps/+; OK107/+, N = 7), chico 1 (chico 1 ; UAS-Epac1-camps/+; OK107/+, N = 8) and rut rescue (chico 1 , UAS-rut/chico 1 ; UAS-Epac1-camps/+; OK107/+, N = 6) brains. Scale bar, 10 μm. f Summary of maximum CFP/YFP ratios upon forskolin stimulation. N.S. Not Significant, ***P < 0.001, **P < 0.01. Data are shown as means ± SEM