Fig. 3

Lower Gfap-positive cell counts in hypothalami of adult Gnasxl ^m+/p- mice. IHC for Gfap on coronal brain sections from WT (a, c, e) and KO (b, d, f) littermates at different rostro-caudal levels. In addition to astrocytes, the α-subset of ependymal tanycytes and their processes (white arrows) were found to express Gfap. a and b Anterior-dorsal hypothalamus at the level of the Paraventricular Nucleus (PVH). c and d Central-ventral hypothalamus at the level of the Arcuate Nucleus (Arc). e and f Level of the posterior hypothalamus (PH). Scale bar = 100 μm. g Quantification of Gfap-expressing cells at the anterior-central and posterior levels, respectively, (anterior & central: WT = 1110 ± 42 vs KO = 937 ± 35 cells/section ± sem, **p < 0.01, t-test, n = 11 matched pairs of sections from 5 WT and 5 KO mice; posterior: WT = 305 ± 21 vs KO = 179 ± 23 cells/section ± sem, ***p < 0.001, t-test, n = 11 matched pairs of sections from 5 WT and 5 KO mice)