Fig. 4
Frizzled and flamingo are required by the ddaE neuron for dendrite directional growth. a Fz is expressed in ddaE neuron in the 3rd instar larva. Fz is labeled by Fz antibody (red) and neurons are labeled by elav-Gal4 (green). White dot lines show the soma of ddaE neuron. b fz expression level is decreased in fz EY03114 mutant. (n = 3). c-d The PD-Angle is affected by Frizzled. The PD-Angle is significantly decreased in fz EY03114 hypomorph mutant and neural fz RNAi expression larvae. Overexpression of Fz in ddaE neuron does not result in an increase of the PD-Angle. Overexpression of Fz in ddaE neuron in fz EY03114 mutant background slightly increased the PD-Angle. (n = 50–79). e-f The PD-Angle is affected by Fmi. Knockdown of fmi in ddaE leads to decreased PD-Angle, and overexpressing Fmi increases it. Neuronal expressing Fmi rescues the decreased PD-Angle in stan f00907 mutant. (n = 26–50). g Knockdown of fz in a stan f00907 mutant background does not further decrease the PD-Angle when compared to stan f00907 mutant or neural knocking down of fz. (n = 39–42). h Overexpression of Fz in a stan f00907 mutant background rescues the decreased PD-Angle in stan f00907 mutant to the wild type degree. (n = 32–49). i PD-Angle of ddaE neuron with neural overexpression of Fmi in fz EY03114 mutant background is comparable to the wild type control. (n = 26–32). Red arrows indicate the initial parts of primary dendrites. Scale bar: 10 μm in (a) and 50 μm in (c)