Fig. 5
Reduction in the Bcl-2 level induced by PAK1-DN is mediated by PAK1-ERK or PAK1 directly. a HEK 293T cells were transfected with 1, 2, or 3 μg of PAK1-DN vectors and with 1 μg of CFP-ERK2 or without CFP-ERK2. Dose-dependent reduction in the Bcl-2 level induced by PAK1-DN was blocked by ERK2 expression. b Bcl-2S87D (serine 87 → aspartic acid), a phosphomimetic form by ERK was resistant to PAK1-DN expression. Bcl-2S87 (serine 87 → alanine) did not show resistance to PAK1-DN expression. Bcl-2S70E (serine 70 → glutamic acid), a putative phosphomimetic form by PAK1 was also resistant to PAK1-DN expression. All Bcl-2 mutants were not altered by PAK1-CA expression. c HEK 293T cells were transfected with FLAG-tagged Bcl-2 and Myc-tagged PAK1-CA or PAK1-DN vectors. The cell lysates were immunoprecipitated with anti-FLAG antibody and the resulting immunoprecipitates were subjected to Western blot analysis using anti-Myc antibody