Fig. 4
From: rAAV-compatible MiniPromoters for restricted expression in the brain and eye
Two MiniPromoters selected based on icre expression, recapitulated their unique expression related to their source gene when tested driving EmGFP. Ple67-EmGFP ± WPRE (FEV RRs) drove expression in the dorsal raphe nuclei of the mouse brain. Ple155-EmGFP ± WPRE (PCP2 RRs) drove expression in the Purkinje cells of the cerebellum, similar to the endogenous gene. In both cases, the presence of WPRE enhanced the number of cells and intensity of cells showing expression. Cb, cerebellum; EmGFP, emerald green fluorescent protein; Raphe, raphe nuclei; RRs, regulatory regions; WPRE, woodchuck hepatitis virus post-transcriptional regulatory element. Blue, Hoechst 33342; Green, EmGFP immunofluorescence. [Scale bars = 200 μm]