Fig. 5

Two CLDN5 RRs-based MiniPromoters (MiniPs) showed brain endothelial cell-specific expression related to their source gene, when virus was introduced into the adult circulatory system. a The Ple34 MiniP drove expression of the miniSOG fluorescent green reporter in blood vessels in the brain. Co-labelling was observed with CD31, a marker of endothelial cells. No miniSOG expression was detected in the heart where CD31 stains endocardial endothelium, or liver, where CD16/32 stains endothelial cells. b Ple261, a “cut down” of Ple34, also drove expression of EGFP in blood vessels in the brain. Again, co-labelling was observed with CD31, a marker of endothelial cells, but no expression was detected in the heart or liver. bp, base pairs; Ctx, cortex; EGFP, enhanced green fluorescent protein; miniSOG, mini Singlet Oxygen Generator; OB, olfactory bulb; RRs, regulatory regions. [Scale bars = 50 μm]