Fig. 1

APLP2 protein and mRNA expression in postnatal developing mouse retinas. a Schema of developmental sequence in the mouse retina. Longitudinal cryostat sections of WT (b-e) and APLP2-KO (e) retinas were immunostained with anti-APLP2 antibody at early postnatal (PN) developmental stages; (b) PN1, (c) PN5, and (d) PN10 and in the adult (e) 3 months-old (3 M). APLP2 was expressed through the different waves of retinal differentiation. G: ganglion cells, AII: AII amacrine cells, S: starburst amacrine cells, P: photoreceptors, H: horizontal cells, R: rods, C: cones, CB: cone bipolar cells, RB: rod bipolar cells, NBL: neuroblastic retina layer, RGCs: retinal ganglion cells, GCL: ganglion cell layer, INL: inner nuclear layer, ONL: outer nuclear layer, IPL: inner plexiform layer, OPL: outer plexiform layer, IS: inner segment, OS: outer segment, RPE: retinal pigment epithelium, E: embryonic stage, PN: postnatal stage. Scale bar 50 μm. (F) Total RNA extracted from WT retinas at the different ages indicated. APLP2 and β-actin mRNA levels visualized by ethidium bromide staining and quantified by RT-PCR. mRNA levels for APLP2 are standardized to β-actin content from the same RNA sample. Means ± s.e.m., N = 5 mice per age group