Skip to main content
Fig. 4 | Molecular Brain

Fig. 4

From: Presynaptic muscarinic acetylcholine autoreceptors (M1, M2 and M4 subtypes), adenosine receptors (A1 and A2A) and tropomyosin-related kinase B receptor (TrkB) modulate the developmental synapse elimination process at the neuromuscular junction

Fig. 4

Involvement of adenosine receptors (AR) and TrkB receptors in axonal elimination. The figure shows the percentage of the singly-, dually- and triply- (or more) innervated NMJs in the YFP control mice exposed to PBS, and after 2 (P7 in a), 4 (P9, in b) and in some cases 10 (P15, in c) applications (one application every day after P5) of the BDNF-TrkB blocking pathway agent TrkB-Fc to sequester endogenous BDNF/NT-4 neurotrophins and the AR pan-inhibitor 8SPT and the AR agonist (ago.) ADO. We also studied axonal elimination after selectively blocking A1R with the antagonist (ant.) DPCPX and inhibiting A2AR with SCH-58261. The control for these selective inhibitors was PBS + DMSO (not shown in the figure) which shows no differences from PBS used as a control by itself. Fisher’s test * p < 0,05, ** p < 0,01, *** p < 0,005

Back to article page

Molecular Brain

ISSN: 1756-6606