Fig. 2
From: Astrocyte-secreted thrombospondin-1 modulates synapse and spine defects in the fragile X mouse model
Quantification of excitatory synapse formation at 17 DIV. a Representative images of WT and Fmr1 KO neurons visualized using immunofluorescence. Inset is an example of a 50 μm segment used for excitatory synapse quantification. b Excitatory synapses are denoted by the arrows representing the co-localization of pre-(synaptophysin, SYN, 1:250, green) and post-synaptic (PSD-95, 1:200, red) puncta (arrows). Synapses were identified using a custom written plug-in for ImageJ (https://imagej.nih.gov/ij/) – “Puncta Analyzer”. c At 17 DIV, Fmr1 KO neurons exhibit a reduction in the number of co-localized puncta relative to their WT counterparts. Data was analyzed by Student’s t-test. Data are presented as the mean ± SEM, *p < 0.05; n = 75 neurons per group, N = 3 independent experiments