Fig. 6

CD mice present decreased levels of BDNF in the hippocampal region. Representative immunofluorescence coronal sections through CA1 area of the hippocampus. BDNF fluorescence in pyramidal cells was measured with ImageJ software and then normalized to MAP2 intensity. 2-4 different regions per mouse were analyzed. BDNF levels in the soma of CA1 and CA3 neurons of CD mice were decreased compared to WT mice (p = 0.04 in CA1 and p = 0.0002 in CA3, unpaired t test). WT: n = 3, CD: n = 2. Data are presented as the mean ± SEM. p values are shown with asterisks indicating values that are significantly different. *p < 0.05; ***p < 0.001