Fig. 4
From: In vivo whole-cell recording with high success rate in anaesthetized and awake mammalian brains
Achieving perforated whole-cell recordings from anaesthetized rats. (a) Perforated whole-cell recordings obtained in layer II/III of the visual cortex. Pipette resistances (monitored in voltage-clamp mode with the application of positive current pulses, as indicated in a5) before (a1, a2) and after (a3) the pipette had made contact with the neuronal membrane during searching for neurons, and pipette resistance after making seals (a4; the pipette potential was hyperpolarized to –70 mV for further seal formation). In (a3), note the AP-like inward currents (as arrowed) before making seals. The depths of the pipette tip beneath the cortical surface are indicated. (b) Spontaneous membrane-potential changes and spike activity of two example visual cortical neurons (in layer II/III) recorded in current-clamp mode (without current injection). The resting potential (RP; with a –13 mV liquid junction potential corrected) is indicated for each cell. (c, d) As in (a, b), for recordings in hippocampal CA1 pyramidal layers. Shadowed areas in (d) are displayed with two different scales