Fig. 2
From: PERK regulates Gq protein-coupled intracellular Ca2+ dynamics in primary cortical neurons
IP3-AM induced intracellular Ca2+ ([Ca2+]i) rise is impaired by acute PERK inhibition. [Ca2+]i. of primary cortical neurons in response to 1 μM IP3-AM treatment (DMSO n = 48, PI n = 48; *** p < 0.001, two-tailed student’s t-Test). Cells were pretreated with 500 nM PERK inhibitor (PI) or DMSO for 15 min before recording. In the representative graph on the left, each Ca2+ trace represents the average of 12–14 neurons that were imaged from the same coverslip. Basal Ca2+ oscillation over 100 sec before treatment and IP3-AM-stimulated [Ca2+]i rise over 600 sec were quantified by calculating the area under the curve (AUC). Final analysis is presented as AUC/100 sec and shown in the bar graph on the right