Fig. 2

DISC1 L100P homozygous mutant shows impaired object recognition memory which is reversed by Clozapine. a Novel objects recognition (NOR) test. Left, L100P mutants and WT mice spent similar time exploring the two objects during test. Right, the percentage of exploration time during NOR test at 1 h after training. WT mice (n = 20) spent significantly more time exploring the novel object versus the old object; while L100P mice (n = 19) did not. Two-way ANOVA and Bonferroni post-tests, ***P < 0.001. b Object-place recognition (NPR) test. Left, L100P mutants and WT mice spent similar time exploring the two objects during test. Right, the percentage of exploration time during NPR test at 24 h after training. WT mice (n = 21) spent significantly more time exploring the object at the new location versus the object at the old location; while L100P mice (n = 19) did not. Two-way ANOVA and Bonferroni post-tests, ***P < 0.001. c Clozapine treatment rescued new object recognition deficits in L100P mice. Left, total objects exploration time in NOR test. Right, the percentage of exploration time during NOR test at 1 h after training. L100P mice treated with clozapine (L100P-cloz, n = 10) spent significantly more time exploring the novel object versus the old object, while L100P mice received vehicle administration (L100P-veh, n = 9) did not. Two-way ANOVA and Bonferroni post-tests, ***P < 0.001. WT-veh n = 10, WT-cloz n = 10. d Clozapine treatment rescued object-place recognition deficits in L100P mice. Left, total objects exploration time in NPR test. Right, the percentage of exploration time during NPR test at 24 h after training. L100P mice treated with clozapine (L100P-cloz, n = 10) spent significantly more time exploring the object at the new location versus the object at the old location, while L100P mice received vehicle administration (L100P-veh, n = 7) did not. Two-way ANOVA and Bonferroni post-tests, **P < 0.01 and ***P < 0.001. WT-veh n = 8, WT-cloz n = 10. All data are shown as means ± SEM.