Fig. 2

ApCPEB4 expression is increased by the activation of 5-HT signaling in the isolated neurites. a ApCPEB4 3′UTR enhances local translation of reporter cDNA. The reporter gene nGFP (nuclear GFP) expression, which normally occurs in the nucleus (control), was observed at the distal neurite in the presence of the 3′UTR of ApCPEB4 (+ApCPEB4-3′UTR). b Immunostaining for ApCPEB4 showed significant induction of ApCPEB4 following 5-HT application in the isolated neurites. The induction of ApCPEB4 was blocked by concurrent treatment of emetine, not by actinomycin D (actD). *, p < 0.05; n.s., not significant, one-way ANOVA; F = 12.73, Tukey’s post-hoc test. c Concurrent treatment of KT5720, a PKA inhibitor, significantly blocked the induction of ApCPEB4 following 5-HT treatment, while the rapamycin (rapa), a blocker for mTOR-dependent protein translation, has no effect on the ApCPEB4 induction. **, p < 0.05; ***, p < 0.001; one-way ANOVA; F = 9.23, Tukey’s post-hoc test