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Fig. 7 | Molecular Brain

Fig. 7

From: CHD7 promotes proliferation of neural stem cells mediated by MIF

Fig. 7

Functional analysis of CHD7 in glioma tumor initiating cells. a, Relative gene expression level of CHD7 was evaluated in GICs, NSPCs and glioma cell compared to astrocytes by qRT-PCR. b, CHD7 supports cell proliferation and/or survival in GICs (GICs-1). Lentiviral CHD7-shRNA expression significantly reduced cell proliferation and/or survival in GICs 5 days after infection. ce, Gene expression of cell cycle arrest genes (c, p21; D, p27) was up-regulated and N-MYC was down-regulated (e) by lentiviral CHD7-shRNA gene silencing in GICs 5 days after infection. Error bars indicate S.D. values; *P < 0.05, **P < 0.01 versus control (n = 3); Student’s t-test. The protein level of p21 (c), p27 (d), and N-MYC (e) was quantified using Image J software, and relative protein levels (normalized by β-actin) are shown above the panel

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