Fig. 7
From: CHD7 promotes proliferation of neural stem cells mediated by MIF
Functional analysis of CHD7 in glioma tumor initiating cells. a, Relative gene expression level of CHD7 was evaluated in GICs, NSPCs and glioma cell compared to astrocytes by qRT-PCR. b, CHD7 supports cell proliferation and/or survival in GICs (GICs-1). Lentiviral CHD7-shRNA expression significantly reduced cell proliferation and/or survival in GICs 5 days after infection. c–e, Gene expression of cell cycle arrest genes (c, p21; D, p27) was up-regulated and N-MYC was down-regulated (e) by lentiviral CHD7-shRNA gene silencing in GICs 5 days after infection. Error bars indicate S.D. values; *P < 0.05, **P < 0.01 versus control (n = 3); Student’s t-test. The protein level of p21 (c), p27 (d), and N-MYC (e) was quantified using Image J software, and relative protein levels (normalized by β-actin) are shown above the panel