Fig. 3

Transduced Tat-HSP22 protein inhibits H₂O₂-induced HT-22 cell death. a Cellular distribution of transduced Tat-HSP22 protein was analyzed using confocal microscopy. HT-22 cells were pretreated with 5 μM of Tat-HSP22 or control HSP22 protein for 2 h and the cells were examined by Histidine and DAPI staining. Scale bar = 20 μm. b The cells were pretreated with Tat-HSP22 or control HSP22 protein (5 μM) for 2 h and the cells were incubated in the absence or presence of H₂O₂ (1 mM) for 12 h. Cell viability was determined using WST-1 assay. ^** P < 0.01 compared with H₂O₂-treated cells