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Fig. 5 | Molecular Brain

Fig. 5

From: Tat-HSP22 inhibits oxidative stress-induced hippocampal neuronal cell death by regulation of the mitochondrial pathway

Fig. 5

Tat-HSP22 protein protects against changes to H2O2-induced mitochondrial membrane potential and cytochrome c release. HT-22 cells were pretreated with 5 μM of Tat-HSP22 or control HSP22 protein for 2 h and exposed to H2O2 (1 mM) for 20 min and 4 h, respectively. a Changes of mitochondrial membrane potential were measured using JC-1 assay kit. Scale bar = 50 μm. b After mitochondria and cytosol fraction were separated using mitochondria isolation kit, transduced Tat-HSP22 protein and cytochrome c levels were determined using Western blotting in each fraction and band intensity by densitometer. ** P < 0.01 compared with H2O2-treated cells

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Molecular Brain

ISSN: 1756-6606