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Fig. 1 | Molecular Brain

Fig. 1

From: Proteasome impairment in neural cells derived from HMSN-P patient iPSCs

Fig. 1

Generation of iPSCs and spinal MN differentiation. a iPSCs were generated from healthy control individuals and HMSN-P patients with TFG P285L mutation. Control and HMSN-P patient iPSCs were morphologically identical to human ESCs and expressed the pluripotent stem cell markers NANOG and SSEA4. Nucleus was stained with DAPI. Scale bars = 200 μm. Differentiated MNs were stained with neuronal marker βIII-tubulin, MAP2, and spinal MN marker SMI-32. Glial cells were stained with GFAP. Scale bars = 50 μm. b - e Proportions of control and HMSN-P patient neurons stained positive for βIII-tubulin (b), MAP2 (c), SMI-32 (d), and GFAP (e) (n = 3, n.s. by Student t-test). There were no significant differences between control and HMSN-P groups. Error bars are ± s.e.m., n.s.: not significant

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