Fig. 3

Immature-like functional properties of GC soma and output synapse after ECS treatment. a, Membrane potential changes (upper) induced by depolarizing currents (lower) in GCs. Scale bars: 100 ms, 20 mV, 40 pA. b, Left: the threshold current intensity required to evoke a single spike (t ₍₅₃₎ = 2.157, * P =0.0356). Center: resting membrane potential (t ₍₄₈₎ = 3.475, ** I = 0.0011, Student’s t-test with Welch’s correction). Right: input resistance. The number (n) represents the number of cells. c, A diagram showing the electrode arrangement for recording field EPSPs at the MF-CA3 synapse. d, No significant change in the input-output relationship at the MF synapse. Left: The relationship between field EPSP and fiber volley amplitude was examined by changing stimulus intensities. Right: The slope value of regression line is shown (t ₍₁₀₎ = 0.9684, P = 0.3557). The n number represents the number of slices. e, No significant change in MF field EPSP to fiber volley ratios at the baseline stimulus intensity (t ₍₁₀₎ = 0.9324, P = 0.3731). f, Reduction of 1-Hz frequency facilitation at the MF synapse after repeated ECS. Inset: sample recordings of MF field EPSPs during baseline and 1-Hz stimulation. Scale bars: 10 ms, 0.5 mV. g, Reduction of frequency facilitation at the MF synapse after ECS repeated twice or more times (ECS × 2, t ₍₇₎ = 4.364, ** P = 0.0033; ECS × 11, t ₍₁₄₎ = 8.832, *** P < 0.001). Both individual (grey) and mean data (red) are shown for ECS-treated groups. The number (n) is shown in the graph. Data are presented as means ± SEM